Widespread distribution of free d‐aspartate in rat periphery

FEBS Letters

Takahashi, Kiyohisa¹  Nishikawa, Toru¹  Hashimoto, Atsushi³  Hayashi, Tokishi²  Oka, Takae¹ 

[1] Department of Mental Disorder Research, National Institute of Neuroscience, Kodaira-shi, Tokyo 187, Japan;Grelan Pharmaceutical Co. Ltd., 2-12-3, Sakurashinmachi, Setagaya-ku, Tokyo 154, Japan;Group of Cell and Information, Precursory Research for Embryonic Science and Technology (PRESTO), Research Development Corporation of Japan, 2-5-2, Nagata-cho. Chiyoda-ku, Tokyo 100, Japan

关键词: d-Aspartate;    d-Aspartate oxidase;    d-Serine;    d-Enantiomer;    Rat periphery;    Boc-L-Cys;    N-tert-butyloxycarbonyl-l-cysteine;    CNS;    central nervous system;    D-HCA;    d-homocysteic acid;    EI;    electron-impact ionization;    GC;    gas chromatography;    HPLC;    high-performance liquid chromatography;    MS;    mass spectrometry;    NMDA;    N-methyl-d-aspartate;    OPA;    o-phthaldialdehyde;    PFPA;    pentafluoropropionic anhydride;    TCA;    trichloroacetic acid;   

DOI  :  10.1016/0014-5793(93)80286-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have identified and quantified free d-aspartate in adult rat peripheral organs using gas chromatographic-mass spectrometric and high-performance liquid Chromatographic techniques. The level of free d-aspartate was highest in the adrenal, testis, spleen and pituitary, followed by the thymus, lung, ovary, placenta, pancreas and heart, and below the detection limit in the kidney, liver, brain, muscle and serum. These data provide the first evidence that a high level of free d-aspartate widely occurs in the adult rat periphery and suggest that the d-amino acid may be an endogenous substrate for d-aspartate oxidase.

【 授权许可】

Unknown