Cloning of a human ether‐a‐go‐go potassium channel expressed in myoblasts at the onset of fusion

FEBS Letters

Sinnreich, Michael²  Liu, Jian-Hui¹  Occhiodoro, Teresa¹  Bader, Charles R¹  Bijlenga, Philippe¹  Fischer-Lougheed, Jacqueline¹  Bernheim, Laurent¹ 

[1] Département de Physiologie, Centre Médical Universitaire (C.M.U.), Division de Recherche Clinique Neuro-Musculaire, Hôpital Cantonal Universitaire, 1 rue Michel-Servet, 1211 Geneva 4, Switzerland;Friedrich Miescher Institut, Basel, Switzerland

关键词: Human EAG;    Potassium channel;    Myoblast fusion;   

DOI  :  10.1016/S0014-5793(98)00973-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

An early sign of human myoblast commitment to fusion is the expression of a non-inactivating delayed rectifier K⁺ current, I_K(NI), and an associated membrane potential hyperpolarization. We have isolated the full-length coding region of a human ether-a-go-go K⁺ channel (h-eag) from myoblasts undergoing differentiation. The h-eag gene was localized to chromosome 1q32–41, and is expressed as a ∼9 kb transcript in myogenic cells and in adult brain tissue. Forced expression of h-eag in undifferentiated myoblasts generates a current with remarkable similarity to I_K(NI) indicating that h-eag constitutes the channel responsible for this current in vivo.

【 授权许可】

Unknown