Functional characterization of ORCTL2 ‐ an organic cation transporter expressed in the renal proximal tubules

FEBS Letters

Housman, David¹  Landers, John¹  Pelletier, Jerry²  Reece, Mark²  Gros, Philippe²  Prawitt, Dirk³  Kast, Christina²  Zabel, Bernhard U.³ 

[1] Center for Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA;Department of Biochemistry, McGill University, Rm 902, 3655 Drummond St., Montreal, Que. H3G 1Y6, Canada;Department of Pediatrics, University of Mainz, Langenbeckstrasse 1, D-55101 Mainz, Germany

关键词: Beckwith-Wiedemann syndrome;    Organic cation transporter;    Tetracycline/H+ antiporter;    Organic cation transporter like-2;    11p15.5;   

DOI  :  10.1016/S0014-5793(98)00907-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Chromosome 11p15.5 harbors a gene or genes involved in Beckwith-Wiedemann syndrome that confer(s) susceptibility to Wilms' tumor, rhabdomyosarcoma, and hepatoblastoma. We have previously identified a transcript at 11p15.5 which encodes a putative membrane transport protein, designated organic cation transporter-like 2 (ORCTL2), that shares homology with tetracycline resistance proteins and bacterial multidrug resistance proteins. In this report, we have investigated the transport properties of ORCTL2 and show that this protein can confer resistance to chloroquine and quinidine when overexpressed in bacteria. Immunohistochemistry analyses performed with anti-ORCTL2 polyc.onal antibodies on human renal sections indicate that ORCTL2 is localized on the apical membrane surface of the proximal tubules. These results suggest that ORCTL2 may play a role in the transport of chloroquine and quinidine related compounds in the kidney.

【 授权许可】

Unknown