Asp‐285 of the metal‐tetracycline/H+ antiporter of Escherichia coli is essential for substrate binding

FEBS Letters

Yamaguchi, Akihito¹  Kimura, Tomomi¹ 

[1] Department of Cell Membrane Biology, Institute of Scientific and Industrial Research, Osaka University, Ibaraki, Osaka 567, Japan

关键词: Tetracycline;    Tetracycline/H+ antiporter;    Tet;    Photolabel;    Subtrate binding;   

DOI  :  10.1016/0014-5793(96)00514-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The transposon Tn10-encoded metal-tetracycline/H⁺ antiporter (TetA(B)) was preferentially photolabeled when [³H]tetracycline was irradiated in the presence of energized inverted membrane vesicles containing the TetA protein. The degree of labeling depended on the duration of irradiation and the energization of the membrane. Photolabeling was not observed in vesicles containing the Asp-285 → Asn mutant TetA protein, indicating that Asp-285 participates in the substrate binding or the step(s) prior to substrate binding

【 授权许可】

Unknown