Protein kinase C inhibitors stimulate arachidonic and docosahexaenoic acids release from uterine stromal cells through a Ca2+‐independent pathway

FEBS Letters

Fayard, Jean-Michel¹  Lagarde, Michel¹  Laugier, Christian¹  Birbes, Hélène¹  Pageaux, Jean-François¹ 

[1] Laboratoire de Biochimie et Pharmacologie, INSERM U.352, INSA-Lyon, Bât 406, 69621 Villeurbanne Cedex, France

关键词: Arachidonic and docosahexaenoic acids;    Ca2+-independent PLA2;    PKC inhibitor;    Uterine stromal cell;    PLA2;    phospholipase A2;    cPLA2;    cytosolic PLA2;    iPLA2;    Ca2+-independent PLA2;    AA;    arachidonic acid;    DHA;    docosahexaenoic acid;    MAPK;    mitogen-activated protein kinase;    PKC;    protein kinase C;    PKA;    protein kinase A;    H-7;    [1-(5-isoquinolinesulfonyl)-2-methyl piperazine;    HCl];    H-89;    {N-[2-((p-bromo-cinnamyl)amino)ethyl]-5-isoquinoline sulfonamide;    HCl};    BEL;    bromoenollactone ((E)-6-(bromomethylene)tetrahydro-3-(1-naphthalenyl)-2H-pyran-2-one);    PMA;    phorbol myristate acetate;    EGTA;    [ethylenebis (oxyethylenenitrilo)] tetraacetic acid;    EDTA;    (ethylenediaminetetraacetic acid;    sodium);    BAPTA;    [1;    2-bis(o-aminophenoxy)ethane-N;    N;    N′;    N′-tetraacteic acid;    sodium];    BSA;    bovine serum albumin;    DTT;    dithiothreitol;    DPPC;    1;    2-dipalmitoyl phosphatidylcholine;    PC;    phosphatidylcholine;    PE;    phosphatidylethanolamine;   

DOI  :  10.1016/S0014-5793(98)00869-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The mechanisms underlying arachidonic acid (AA) release by uterine stromal (U_III) cells were studied. Stimulation of AA release by calcium ionophore and PMA are inhibited by various PKC inhibitors and by calcium deprivation. These results suggest the involvement of an AA-specific cPLA₂ as the release of docosahexaenoic acid (DHA) from prelabelled cells is much lower than the release of AA. The results also show a more original stimulation of AA and DHA release induced by PKC inhibitors, which is insensitive to calcium deprivation. This stimulation is not due to acyltransferase inhibition, suggesting the participation of a Ca²⁺-independent PLA₂ (iPLA₂). However, iPLA₂ activity measured in U_III cells is inhibited by the specific iPLA₂ inhibitor, BEL, and is not stimulated by PKC inhibitors, in contrast with the AA and DHA release. It seems therefore that this iPLA₂ cannot be involved in this mechanism. The participation of another iPLA₂, BEL-insensitive, is discussed.

【 授权许可】

Unknown