Low molecular weight phosphotyrosine protein phosphatase translocation during cell stimulation with platelet‐derived growth factor

FEBS Letters

Rigacci, Stefania¹  Bucciantini, Monica¹  Marzocchini, Riccardo¹  Berti, Andrea¹ 

[1] Department of Biochemical Sciences, University of Florence, Viale Morgagni 50, 50134 Florence, Italy

关键词: Phosphotyrosine protein phosphatase;    Signal transduction;    Enzyme translocation;    Platelet-derived growth factor receptor;    PTP;    phosphotyrosine protein phosphatase;    EGFr;    epidermal growth factor receptor;    PDGFr;    platelet-derived growth factor receptor;    SH2;    src-homology type two;    FCS;    fetal calf serum;    PMSF;    phenylmethylsulfonyl fluoride;    BCA;    bicinchoninic acid;    SDS-PAGE;    sodium dodecyl sulfate-polyacrylamide gel electrophoresis;    ECL;    enhanced chemiluminescence;    PBS;    phosphate-buffered saline;   

DOI  :  10.1016/S0014-5793(98)00847-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Low M _r phosphotyrosine protein phosphatase (PTP) is a cytosolic enzyme whose activity upon platelet-derived growth factor (PDGF) and insulin receptors has been demonstrated in vivo. In our study we demonstrate that this enzyme, both naturally expressed and overexpressed in NIH/3T3 fibroblasts, translocates from the cytosol to the Triton X-100 insoluble fraction following stimulation with PDGF. It emerges that the phosphorylation of a defined population of PDGF receptors, which is localized in this fraction and seems to be endowed with peculiar features and functions, is particularly affected by low M _r PTP overexpression.

【 授权许可】

Unknown