FEBS Letters | |
Cloning of murine low molecular weight phosphotyrosine protein phosphatase cDNA: identification of a new isoform | |
Camici, G1  Giannoni, E1  Cirri, P1  Modesti, A1  Paoli, P1  Raugei, G1  Magherini, F1  Ramponi, G1  | |
[1] Dipartimento di Scienze Biochimiche, Università di Firenze, viale Morgagni 50, 50134 Florence, Italy | |
关键词: Phosphotyrosine protein phosphatase; Alternative splicing; Mouse; LMW-PTP; low molecular weight phosphotyrosine protein phosphatase; PTPs; protein tyrosine phosphatases; PTKs; protein tyrosine kinases; ERK; extracellular signal-regulated kinase; STAT; signal transducer and activators of transcription; pNPP; para-nitrophenyl phosphate; RACE; rapid amplification of cDNA ends; | |
DOI : 10.1016/S0014-5793(98)01241-1 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The low molecular weight phosphotyrosine protein phosphatase (LMW-PTP) is a 18 kDa cytosolic enzyme, involved in the negative regulation of cell proliferation. In different mammalian species LMW-PTPs are expressed in two molecular forms produced from a single primary transcript through an alternative splicing mechanism. In this paper we report the cloning, expression and characterization of mouse isoforms of LMW-PTPs (called m-IF1 and m-IF2), very similar to the corresponding rat and human isoenzymes. Moreover we have identified a third cDNA encoding a protein (m-IF2P) that presents three substitutions compared to m-IF2. This new isoform is still active on pNPP, although to a lower extent: this reduction is mainly due to the leucine to proline substitution in position 13, within the catalytic loop. The mRNA expression level of this isoform is comparable to those of m-IF1 and m-IF2. It is likely that a gene duplication process followed by mutations has generated this new gene.
【 授权许可】
Unknown
【 预 览 】
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