Neurotensin type 1 receptor‐mediated activation of krox24, c‐fos and Elk‐1: preventing effect of the neurotensin antagonists SR 48692 and SR 142948

FEBS Letters

Gully, Danièle¹  Combes, Thérèse²  Casellas, Pierre²  Maffrand, Jean-Pierre¹  Portier, Marielle² 

[1] Sanofi Recherche, 195 Route d'Espagne, 31036 Toulouse cedex, France;Sanofi Recherche, 371 rue du Pr. J. Blayac, 34184 Montpellier cedex 04, France

关键词: Neurotensin;    Neurotensin type 1 receptor;    krox24;    c-fos;    Elk-1;    cAMP;    Luciferase;    CHO;    Chinese hamster ovary;    CRE;    cAMP responsive element;    NT;    neurotensin;    NT1-R;    neurotensin type 1 receptor;    NT2-R;    neurotensin type 2 receptor;    MAPK;    mitogen-activated protein kinase;    RLU;    relative light unit;    SRE;    serum response element;    TCF;    ternary complex factor;   

DOI  :  10.1016/S0014-5793(98)00749-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Stimulation of neurotensin (NT) type 1 receptors (NT1-R) in transfected CHO cells is followed by the activation of mitogen-activated protein kinases and the expression of the early response gene krox24. By making point mutations and internal deletions in the krox24 promoter, we show that proximal serum responsive elements (SRE) are involved in transcriptional activation by NT. In addition, we show that the related early response gene c-fos and the Ets protein Elk-1 are also induced by NT. The involvement of NT1-R in NT-mediated activation of krox24, c-fos and Elk-1 was demonstrated by the preventing effect of the specific antagonists SR 48692 and SR 142948. Finally, we show that the activation of krox24 and Elk-1 on the one hand, and that of c-fos on the other hand, result from independent transduction pathways since the former are pertussis toxin-sensitive whereas the latter is insensitive to pertussis toxin.

【 授权许可】

Unknown