[Ser7]neurotensin: isolation from guinea pig intestine

FEBS Letters

Shaw, Christopher²  Thim, Lars¹  Conlon, J.Michael² 

[1] Clinical Research Group for Gastrointestinal Endocrinology of the Max-Planck-Gesellschaft Novo Research Institute, Bagsvaerd, Denmark;Clinical Research Group for Gastrointestinal Endocrinology of the Max-Planck-Gesellschaft, University of Göttingen, Goßlerstraße 10d, D-3400 Göttingen, FRG

关键词: Neurotensin;    (Guinea pig);    Gastrointestinal peptide;    Primary structure;    HPLC;    Radioimmunoassay;   

DOI  :  10.1016/0014-5793(86)80684-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Using three antisera to neurotensin of defined regional specificity, a novel neurotensin has been identified in extracts of guinea pig brain and small intestine. The primary structure of the peptide was established as: pGlu Leu Tyr GIu Asn Lys Ser Arg Arg Pro Tyr He Leu. Guinea pig neurotensin differs from bovine neurotensin by substitution of a prolyl residue by a seryl residue at position 7. Synthetic [Ser⁷]neurotensin showed identical Chromatographie and immunochemical properties to guinea pig neurotensin. This difference in primary structure may account for some of the anomalous pharmacological effects of bovine neurotensin on guinea pig tissues.

【 授权许可】

Unknown