Genomic organization and promoter function of the mouse uncoupling protein 2 (UCP2) gene

FEBS Letters

Satoh, Teturou¹  Mori, Masatomo¹  Iwasaki, Toshiharu¹  Monden, Tsuyoshi¹  Yamada, Masanobu¹  Murakami, Masami¹  Hashida, Tetsu¹  Shibusawa, Nobuyuki¹ 

[1] First Department of Internal Medicine, Gunma University School of Medicine, 3-39-15 Showa-machi, Maebashi, Gunma 371-8511, Japan

关键词: Uncoupling protein 2;    Gene;    Promoter;   

DOI  :  10.1016/S0014-5793(98)00835-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We cloned and characterized the mouse uncoupling protein 2 (UCP2) gene and its promoter region. The gene spans approximately 6.3 kb and contains eight exons and seven introns. Two short exons are located in the 5′ untranslated region, and each of the remaining exons encodes one of the transmembrane domains. 3′-RACE analysis showed that a polyadenylation signal 257 bp downstream from the stop codon was functional. Primer extension analysis indicated a single transcriptional start site 369 bp upstream from the translational start site. The promoter region lacks both TATA and CAAT boxes but is GC-rich. A construct containing 1250 bp of the promoter region showed significant activity in all 6 cell lines examined, and the region between −160 and −678 bp exhibited strong positive regulatory activity. These features of the UCP2 gene are different from those of the UCP1 gene and may contribute to its ubiquitous expression.

【 授权许可】

Unknown