Genomic organization of the KTX2 gene, encoding a ‘short’ scorpion toxin active on K+ channels

FEBS Letters

Bougis, Pierre E¹  Martin-Eauclaire, Marie-France¹  Legros, Christian¹ 

[1] Laboratoire de Biochimie, UHR 6560 du CNRS, Ingénierie des Protéines, Institut Fédératif INSERM Jean Roche, Faculté de Médecine Nord, Université de la Méditerranée, Bd Pierre Dramard, F-13916 Marseille, Cedex 20, France

关键词: Scorpion toxin;    Potassium channel;    Gene;    Promoter;   

DOI  :  10.1016/S0014-5793(96)01492-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A single intron of 87 bp, close to the region encoding the C-terminal part of the signal peptide, was found in the gene of the ‘short’ scorpion toxin kaliotoxin 2 of Androctonus australis acting on various types of K⁺ channels. Its A+T content was particularly high (up to 86%). By walking and ligation-mediated PCR, the promoter sequences of the kaliotoxin 2 gene of Androctonus australis were studied. The transcription unit of the gene is 390 bp long. Consensus sequences were identified. The genes of ‘short’ scorpion toxins active on K⁺ channels are organized similarly to those of the ‘long’ scorpion toxins active on Na⁺ channels and not like those of structurally related insect defensins, which are intronless.

【 授权许可】

Unknown