Identification and characterisation of a novel splice variant of synaptojanin1

FEBS Letters

Parker, Peter J¹  Radley, Elisabeth¹  Finan, Peter M¹  Woscholski, Rüdiger¹ 

[1] Protein Phosphorylation Laboratory, Imperial Cancer Research Fund, P.O. Box 123, 44 Lincoln's Inn Fields, London WC2A 3PX, UK

关键词: Splice variant;    Synaptojanin;    Inositol;    5-Phosphatase;    Lipid;   

DOI  :  10.1016/S0014-5793(98)00820-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Synaptojanin1, the major constitutively active PtdInsP₃ 5-phosphatase activity in rat brain, is one of two closely related proteins both extensively spliced in their C-terminal proline rich domain. We describe here the discovery of a novel splice variant of synaptojanin1 which misses the major N-terminal part of the SAC1 domain. This ΔSAC-synaptojanin1 is expressed in rat brain tissue as shown by Northern and Western analysis. However, the deletion of the SAC1 domain does not alter PtdInsP₃ 5-phosphatase activity demonstrating that the SAC1 domain is not necessary for catalytic function.

【 授权许可】

Unknown