Targeting signals for a bacterial Sec‐independent export system direct plant thylakoid import by the ΔpH pathway

FEBS Letters

Robinson, Colin²  Klösgen, Ralf Bernd⁴  Wexler, Margaret³  Berks, Ben C.³  Bogsch, Erik G.²  Palmer, Tracy¹ 

[1] School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, UK;Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, UK;Centre for Metalloprotein Spectroscopy and Biology, University of East Anglia, Norwich NR4 7TJ, UK;Botanisches Institut der Ludwig-Maximilians-Universität, Menzinger Strasse 67, 80638 Munich, Germany

关键词: Sec-independent;    Bacterial protein export;    Thylakoid import;    Twin-arginine transfer peptide;    Signal peptide;   

DOI  :  10.1016/S0014-5793(98)00790-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Preproteins targeted to the Sec-independent protein transport systems of plant thylakoids and of bacteria both have unusual transfer peptides bearing a consensus twin-arginine motif. Possible mechanistic similarity between the two Sec-independent transport pathways was investigated by assessing the ability of bacterial twin-arginine transfer peptides to direct thylakoid import. High efficiency import was observed. This process was demonstrated to occur specifically via the Sec-independent ΔpH pathway and to depend on an intact twin-arginine motif on the transfer peptide. These results provide strong evidence for the operation of mechanistically related Sec-independent protein transport pathways in chloroplasts and bacteria.

【 授权许可】

Unknown