Identification of a cytochrome P450 cDNA encoding (2S)‐flavanone 2‐hydroxylase of licorice (Glycyrrhiza echinata L.; Fabaceae) which represents licodione synthase and flavone synthase II

FEBS Letters

Ayabe, Shin-ichi¹  Akashi, Tomoyoshi¹  Aoki, Toshio¹ 

[1] Department of Applied Biological Science, Nihon University, Fujisawa, Kanagawa 252-8510, Japan

关键词: Cytochrome P450;    Elicitation;    (2S)-flavanone 2-hydroxylase;    Flavonoid biosynthesis;    Heterologous expression;    Glycyrrhiza echinata;    F2H;    (2S)-flavanone 2-hydroxylase;    FNSII;    flavone synthase II;    LS;    licodione synthase;    P450;    cytochrome P450;   

DOI  :  10.1016/S0014-5793(98)00781-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The microsome of insect cells expressing CYP Ge-5 (CYP93B1), a cytochrome P450 cDNA of licorice (Glycyrrhiza echinata L.), catalyzed the formation of [¹⁴C]licodione and [¹⁴C]-2-hydroxynaringenin from (2S)-[¹⁴C]liquiritigenin and (2S)-[¹⁴C]naringenin, respectively. On acid treatment, the products were converted to ¹⁴C-labeled 7,4′-dihydroxyflavone and apigenin. Eriodictyol was also converted to luteolin by the reaction with the microsome of yeast expressing CYP93B1 and subsequent acid treatment. CYP93B1 was thus shown to encode (2S)-flavanone 2-hydroxylase, which has previously been designated to licodione synthase and flavone synthase II depending on the substrates employed.

【 授权许可】

Unknown