Antioxidant enzymatic activities and lipid peroxidation in cultured human chondrocytes from vertebral plate cartilage

FEBS Letters

Gaetani, Paolo³  Marzatico, Fulvio²  Schiavo, Roberta¹  Grazioli, Vittorio¹  Rodriguez y Baena, Riccardo³  Casari, Erminia¹ 

[1] Department of Laboratory Medicine, Istituto Clinico Humanitas, 20089 Rozzano, Italy;Neurosurgery Unit, Istituto Clinico Humanitas, 20089 Rozzano, Italy;Institute of Pharmacology, University of Pavia, Pavia, Italy

关键词: Human chondrocyte;    ROS;    Superoxide dismutase;    Catalase;    Glutathione peroxidase;    ROS;    reactive oxygen species;    PBS;    phosphate buffered saline;    HBSS;    Hank's balanced salt solution;    FCS;    fetal calf serum;    LDH;    lactate dehydrogenase;    GSSG;    glutathione (oxidized form);    SOD;    total superoxide dismutase;    CAT;    catalase;    GSHPx;    glutathione peroxidase;   

DOI  :  10.1016/S0014-5793(98)00731-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Total SOD activity increased, even if not significantly, between the 12th and the 18th day. A significant variation of GSHPx (P<0.01) and of catalase (P<0.001) activity was observed between the 3rd and the 6th day with no further variation until the 18th day. A significant increase (P<0.001) of lipid peroxidation from the 3rd to the 18th day was also observed. These results seem to indicate that only fresh human cultured chondrocytes are suitable to study, through in vitro models, the in vivo behavior of the antioxidative status of these cells.

【 授权许可】

Unknown