Different effects of an oligonucleotide uptake stimulating protein on leukemic cells in their primitive and differentiated state

FEBS Letters

Rák, Kálmán²  Szegedi, Gyula³  Kiss, Attila²  Sipka, Sándor³  Tóth, György¹  Schlammadinger, József¹  Szabó, Gábor¹ 

[1] Department of Biology, University Medical School of Debrecen, P.O. Box 1, H-4012 Debrecen, Hungary;2nd Department of Internal Medicine, University Medical School of Debrecen, P.O. Box 1, H-4012 Debrecen, Hungary;3rd Department of Internal Medicine, University Medical School of Debrecen, P.O. Box 1, H-4012 Debrecen, Hungary

关键词: Oligonucleotide uptake;    DNA uptake stimulating protein;    Leukemia;    Cell proliferation;    Antisense oligonucleotide;    Ara-C;    cytosine-β-d-arabinofuranoside;    B-CLL;    B-cell chronic lymphoid leukemia;    CGL;    chronic granulocytic leukemia;    DNase I;    deoxyribonuclease I (EC 3.1.21.1);    DUSF;    DNA uptake stimulating factor;    HU;    hydroxyurea;    PHA;    phytohemagglutinin;    ss;    single stranded;    TPA;    12-O-tetradecanoyl-phorbol-13-acetate;    WBC;    white blood cell;   

DOI  :  10.1016/S0014-5793(98)00663-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The single stranded [³H]oligonucleotide uptake by HL-60 human promyelocyte and K562 human erythroleukemia cells was stimulated 20–45-fold by DUSF (DNA uptake stimulating protein), and this effect was drastically reduced (to 1.6–13×) if the cells were induced to differentiate. The oligonucleotide uptake stimulating effect of DUSF was not altered in HL-60 and K562 cells, if the proliferation of the cells was inhibited by hydroxyurea (HU) treatment. The oligonucleotide uptake by separated granulocytes and mononuclear cells from healthy donors was not stimulated by DUSF, while the uptake of oligonucleotides by myeloid and lymphoid leukemic cells was greatly stimulated (10–15×). The uptake of oligonucleotides by differentiated mononuclear cells of healthy donors could not be stimulated by DUSF, but the oligonucleotide uptake was greatly increased (11×) by DUSF if the cells were subjected to blast transformation.

【 授权许可】

Unknown