Identification of sucrose synthase as an actin‐binding protein

FEBS Letters

Winter, Heike¹  Huber, Steven C¹  L. Huber, Joan¹ 

[1] U.S. Department of Agriculture, Agricultural Research Service, Raleigh, NC, USA

关键词: Actin-binding protein;    Actin cytoskeleton;    Aldolase;    Sucrose synthase;    Phalloidin;    SuSy;    sucrose synthase;    Suc;    sucrose;   

DOI  :  10.1016/S0014-5793(98)00659-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Several lines of evidence indicate that sucrose synthase (SuSy) binds both G- and F-actin: (i) presence of SuSy in the Triton X-100-insoluble fraction of microsomal membranes (i.e. crude cytoskeleton fraction); (ii) co-immunoprecipitation of actin with anti-SuSy monoclonal antibodies; (iii) association of SuSy with in situ phalloidin-stabilized F-actin filaments; and (iv) direct binding to F-actin, polymerized in vitro. Aldolase, well known to interact with F-actin, interfered with binding of SuSy, suggesting that a common or overlapping binding site may be involved. We postulate that some of the soluble SuSy in the cytosol may be associated with the actin cytoskeleton in vivo.

【 授权许可】

Unknown