Identification of an actin binding region in aldolase

FEBS Letters

Clarke, Frank¹  O'Reilly, Gabrielle¹ 

[1] Faculty of Science and Technology, Griffith University, Nathan 4111, Australia

关键词: Aldolase;    Actin;    Actin binding peptide;   

DOI  :  10.1016/0014-5793(93)80623-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Fragmentation of the actin binding glycolytic enzyme, aldolase, with cyanogen bromide yields an 18K actin binding fragment which corresponds to residues 1–164 of the aldolase sequence. Within this fragment there is a region of sequence (residues 32–52) which is highly homologous to a region of sequence near the C-terminus of actin itself and which is also found in the actin binding domains of a number of other actin binding proteins. A synthetic peptide corresponding to the aldolase sequence 32–52 encompassing this region of homology binds to F-actin and specifically competes with native aldolase for binding to this cytoskeletal protein.

【 授权许可】

Unknown