Initiation of in vitro reverse transcription from tRNALys3 on HIV‐1 or HIV‐2 RNAs by both type 1 and 2 reverse transcriptases

FEBS Letters

Litvak, Simon¹  Boulmé, Florence¹  Freund, Frédéric¹ 

[1] EP-630, CNRS-Université Victor Ségalen Bordeaux 2, IFR 66 `Pathologies Infectieuses', 1, rue Camille Saint-Saëns, 33077 Bordeaux Cedex, France

关键词: Human immunodeficiency virus type 1;    Human immunodeficiency virus type 2;    tRNALys3;    Reverse transcription;    Initiation;    HIV;    human immunodeficiency virus;    RT;    reverse transcriptase;    PBS;    primer binding site;    ODN;    oligodeoxyribonucleotide;    ORN;    oligoribonucleotide;    LTR;    long terminal repeat;    SIV;    simian immunodeficiency virus;   

DOI  :  10.1016/S0014-5793(98)00649-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

HIV reverse transcription is initiated from a cellular tRNA partially associated with the retroviral genome. Here we studied homologous HIV-2 cDNA synthesis using natural or synthetic primers. With natural tRNA^Lys3, synthesis of early products comprising nucleotides +5 to +7 preceded the elongation step leading to synthesis of (−) strong-stop cDNA. In the presence of a poly(A)·oligo(dT) trap, no full-length product was observed while early products were still present, showing a transition between initiation and elongation. With DNA primers only an unspecific elongation was found. Our data show a similar mechanism of reverse transcription initiation by HIV-1 and HIV-2 reverse transcriptases. Furthermore, using a heterologous system we found that HIV-1 RNA, in contrast to data reported in the literature, was an excellent template for HIV-2 reverse transcriptase.

【 授权许可】

Unknown