One-Step Reverse Transcription Droplet Digital PCR for Quantitative Detection of Porcine Reproductive and Respiratory Syndrome Virus

2018 4th International Conference on Environmental Science and Material Application

生态环境科学;材料科学

Niu, Chunyan^1 ; Yang, Jiayi^1 ; Dong, Lianhua^1 ; Sui, Zhiwei^1 ; Wang, Jing^1

Division of Medical and Biological Measurement, National Institute of Metrology, China^1

关键词: Accurate quantifications;    Clinical disease;    Economic problems;    Quantification methods;    Quantitative detection;    Reverse transcriptases;    Reverse transcription;    Viral detection;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/252/4/042005/pdf
DOI  :  10.1088/1755-1315/252/4/042005

来源: IOP

【 摘 要 】

Porcine reproductive and respiratory syndrome virus (PRRSV) is a single-stranded and positive-sense RNA virus which can cause severe clinical disease and economic problem for worldwide pig industry. Droplet digital PCR (ddPCR) is an absolutely quantification method that can be used in viral detections. Here a one-step reverse transcription ddPCR was established for detection of highly pathogenic PRRSV (HP-PRRSV) and classical PRRSV. The assay was able to quantitatively detect HuN4 from 103 to 10-1 TCID50/mL. Through comparing with real-time quantitative reverse transcriptase PCR, the one-step RT-ddPCR was demonstrated more sensitive and can be used for the accurate quantification of PRRSV.