Immunoglobulin superfamily members gp65 and gp55: tissue distribution of glycoforms

FEBS Letters

Mummery, Rosemary²  Beesley, Philip W.²  Gundelfinger, Eckart D.¹  Langnaese, Kristina² 

[1] Federal Institute for Neurobiology, Brenneckestrasse 6, Postfach 1860, D-39008 Magdeburg, Germany;Division of Biochemistry, School of Biological Sciences, Royal Holloway, University of London, Egham Hill, Egham, Surrey TW20 OEX, UK

关键词: Immunoglobulin superfamily;    Glycosylation;    Glycoform;    Synapse;    Concanavalin A;    Con A;    concanavalin A;    endo-F/PNGase F;    endoglycosidase F/peptide-N glycosidase F;    Ig;    immunoglobulin;    mab;    monoclonal antibody;    PBS;    phosphate-buffered saline;    PSD;    post-synaptic density;    SM;    synaptic membrane;   

DOI  :  10.1016/S0014-5793(98)00616-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Gp65 and gp55 are immunoglobulin superfamily members produced by alternative splicing of the same gene transcript, and originally identified as components of synaptic membranes. A monoclonal antibody specific for gp65 and gp55 has been used to detect immunoreactive species in a wide range of tissues. All immunoreactive species bind to concanavalin A and deglycosylation studies show that in all tissues tested other than brain the immunoreactive species are derived from gp55. HEK cells transfected with gp65 or gp55 express different glycoforms from brain showing that the pattern of glycosylation of these molecules is dependent upon the cell type in which they are expressed.

【 授权许可】

Unknown