The carboxy‐terminal region of adenovirus E1A activates transcription through targeting of a C‐terminal binding protein‐histone deacetylase complex

FEBS Letters

Sundqvist, Anders¹  Sollerbrant, Kerstin²  Svensson, Catharina¹ 

[1]Department of Medical Biochemistry and Microbiology, BMC, Uppsala University, Box 582, S-751 23 Uppsala, Sweden

[2]Center for Genomics Research, Karolinska Institute, S-171 77 Stockholm, Sweden

关键词: Adenovirus E1A;    Transcription;    C-terminal binding protein;    Proliferating cell nuclear antigen;    Histone deacetylase;   

DOI  :  10.1016/S0014-5793(98)00588-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Binding of the C-terminal binding protein, CtBP, to the adenovirus E1A moiety of a Gal4-E1A fusion protein abolishes conserved region (CR) 1-dependent transcription activation. In contrast, a non-promoter targeted E1A peptide, capable of binding CtBP, can induce transcription from the proliferating cell nuclear antigen (PCNA) promoter. CtBP is shown here to bind the histone deacetylase HDAC1, suggesting that a promoter targeted CtBP-HDAC1 complex can silence transcription from the PCNA promoter through a deacetylation mechanism. Expression of the CtBP binding domain of E1A is sufficient to alleviate repression, possibly due to the displacement of the CtBP-HDAC1 complex from the promoter.

【 授权许可】

Unknown