Adenovirus E1A blocks oxidant‐dependent ferritin induction and sensitizes cells to pro‐oxidant cytotoxicity

FEBS Letters

Torti, Suzy V.²  Tsuji, Yoshiaki¹  Torti, Frank M.¹  Orino, Kouichi² 

[1] Department of Cancer Biology, and the Comprehensive Cancer Center, Wake Forest University School of Medicine, Winston-Salem, NC 27157, USA;Department of Biochemistry, and the Comprehensive Cancer Center, Wake Forest University School of Medicine, Winston-Salem, NC 27157, USA

关键词: Ferritin;    Oxidative stress;    Adenovirus E1A;   

DOI  :  10.1016/S0014-5793(99)01443-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Ferritin is a protein that oxidizes and sequesters intracellular iron in a mineral core. We have reported that the E1A oncogene selectively represses ferritin H transcription, resulting in reduced levels of the ferritin H protein. Here we demonstrate that cells respond to pro-oxidant challenge by inducing ferritin mRNA and protein, and that this response is completely blocked by E1A. Concordantly, E1A sensitized cells to the cytotoxic effects of oxidative stress and enhanced the accumulation of reactive oxygen species in response to pro-oxidant challenge. These results demonstrate that expression of E1A impedes the cellular response to oxidative stress, including the induction of ferritin.

【 授权许可】

Unknown