Identification of multispecific organic anion transporter 2 expressed predominantly in the liver

FEBS Letters

Cha, Seok Ho²  Sekine, Takashi¹  Endou, Hitoshi¹  Tsuda, Minoru¹  Apiwattanakul, Nopporn¹  Kanai, Yoshikatsu¹  Nakajima, Noriko¹ 

[1] Kyorin University School of Medicine, Department of Pharmacology and Toxicology, Mitaka, Tokyo 181, Japan;Department of Pharmacology, Catholic University Medical College, Banpo-dong 505, Seocho-gu, Seoul 137-701, South Korea

关键词: Liver;    Multispecific transporter;    Organic anion;    Pharmacokinetics;   

DOI  :  10.1016/S0014-5793(98)00585-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In the present study, we demonstrate that NLT (novel liver-specific transport protein) is a multispecific organic anion transporter of the liver. The amino acid sequence of NLT shows 42% identity to that of the renal multispecific organic anion transporter, OAT1. When expressed in Xenopus laevis oocytes, NLT mediated uptake of organic anions, such as salicylate, acetylsalicylate, PGE₂, dicarboxylates and p-aminohippurate. [¹⁴C]Salicylate uptake via NLT was saturable (K _m=88.8±23.4 μM) and sodium-independent. Expression of the mRNA of NLT was detected in the liver and kidney (liver≫kidney). We propose that NLT be renamed OAT2.

【 授权许可】

Unknown