2‐Arachidonoylglycerol, an endogenous cannabinoid receptor agonist: identification as one of the major species of monoacylglycerols in various rat tissues, and evidence for its generation through Ca2+‐dependent and ‐independent mechanisms

FEBS Letters

Waku, Keizo¹  Tokumura, Akira²  Kodaka, Tomoko¹  Kondo, Sachiko¹  Sugiura, Takayuki¹  Kondo, Hironori¹  Nakane, Shinji¹ 

[1] Faculty of Pharmaceutical Sciences, Teikyo University, Sagamiko, Kanagawa 199-0195, Japan;Faculty of Pharmaceutical Sciences, Tokushima University, Tokushima, Tokushima 770-8505, Japan

关键词: 2-Arachidonoylglycerol;    Monoacylglycerol;    Cannabinoid;    CB1 receptor;    LTP;    long-term potentiation;    BHT;    butylated hydroxytoluene;    DFP;    diisopropylfluorophosphate;    TMS;    trimethylsilyl;    GABA;    γ-aminobutyric acid;    Fatty acids are designated in terms of number of carbon atoms/number of double bonds;    e.g. 20:4 for arachidonic acid;   

DOI  :  10.1016/S0014-5793(98)00581-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The molecular species compositions of monoacylglycerols obtained from various rat tissues were examined by reverse-phase high-performance liquid chromatography (HPLC) and gas chromatography-mass spectrometry (GC-MS) analyses. We confirmed that 2-arachidonoylglycerol, an endogenous cannabinoid receptor agonist, is one of the most abundant molecular species of monoacylglycerols in the brain. Substantial amounts of 2-arachidonoylglycerol were also found in the liver, spleen, lung and kidney, but the levels were considerably lower than that in the brain. We found that a small amount of 2-arachidonoylglycerol was generated in a brain homogenate during incubation in the absence of Ca²⁺. Importantly, the generation of 2-arachidonoylglycerol was markedly augmented in the presence of Ca²⁺, suggesting that Ca²⁺ plays a key role in regulation of the generation of 2-arachidonoylglycerol in this tissue.

【 授权许可】

Unknown