Characterization of a gene encoding an abscisic acid‐inducible type‐2 lipid transfer protein from rice

FEBS Letters

Delseny, Michel¹  Martı́nez-Izquierdo, José Antonio²  Menossi, Marcelo²  Puigdoménech, Pére²  Garcı́a-Garrido, José Manuel¹ 

[1] Laboratoire de Physiologie et Biologie Moléculaire Végétales, UMR 5545 CNRS Université de Perpignan, 66860 Perpignan Cedex, France;Departamento de Genética Molecular, CID-CSIC, Jordi Girona 18, 08034 Barcelona, Spain

关键词: Lipid transfer protein;    Abscisic acid;    Abscisic acid responsive element;   

DOI  :  10.1016/S0014-5793(98)00529-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The cloning and sequence analysis of a novel gene that encodes a type 2 non-specific lipid transfer-like protein (LTP) from rice is reported. Sequence analysis revealed an ORF encoding a protein showing characteristics of the LTP proteins. However, rice LTP2 is more similar to heterologous LTPs than to rice LTP1, supporting the existence of two distinct families of plant LTPs. Ltp2 mRNA is accumulated only in mature seeds. In vegetative tissues, mRNA was only detected after treatment with abscisic acid (ABA), mannitol or NaCl. Transient expression experiments that the 61 nucleotides upstream of the TATA box, containing two ACGT boxes and the motif I, are sufficient for ABA responsiveness of the Ltp gene.

【 授权许可】

Unknown