Lipid transfer proteins (nsLTPs) from barley and maize leaves are potent inhibitors of bacterial and fungal plant pathogens

FEBS Letters

García-Olmedo, Francisco¹  Segura, Ana¹  Molina, Antonio¹ 

[1] Laboratorio de Bioquímica y Biología Molecular, ETS Ingenieros Agrónomes- UP M, E-28040 Madrid, Spain

关键词: Lipid transfer protein;    Plant pathogen;    Thionin;    nsLTP;    non-specific lipid transfer protein;    EDTA;    ethylene-diamine-tetracetic acid;    RP-HPLC;    reverse-phase high-performance liquid chromatography;    SDS;    sodium dodecyl sulfate;    PAGE;    polyacrylamide gel electrophoresis;    SGE;    starch-gel electrophoresis;    cfu;    colony forming units;   

DOI  :  10.1016/0014-5793(93)81198-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Four homogeneous proteins (Cw₁₈, Cw₂₀, Cw₂₁, Cw₂₂) were isolated from etiolated barley leaves by extraction of the insoluble pellet from a Tris-HCl (pH 7.5) homogenate with 1.5 M LiCl and fractionation by reverse-phase high-performance liquid chromatography. All 4 proteins inhibited growth of the pathogen Clavibacter michiganensis subsp. sepedonicus (EC₅₀S = 1−3 × 10⁻⁷ M) and had closely related N-terminal amino acid sequences. The complete amino acid sequences of proteins Cw₁₈ and Cw₂₁ were determined and found to be homologous to previously described, non-specific lipid transfer proteins from plants (32–62% identical positions). The proteins also inhibited growth of the bacterial pathogen Pseudomonas solanacearum (EC₅₀s = 3–6 × 10⁻⁷ M) and the fungus Furium solani (EC₅₀s = 3–20 × 10⁻⁶ M). A homologous protein from maize leaves (Cw₄₁) was purified in a similar manner and also found to have inhibitory properties, A synergistic effect against the fungus was observed when protein Cw₂₁, was combined with thionins. A defense role for non-specific lipid transfer proteins from plants is proposed.

【 授权许可】

Unknown