Requirement of membrane‐proximal amino acids in the carboxyl‐terminal tail for expression of the rat AT1a angiotensin receptor

FEBS Letters

Jagadeesh, Gowraganahalli³  Hunyady, László¹  Gáborik, Zsuzsanna¹  Mihalik, Balázs¹  Catt, Kevin J.²  Jayadev, Suman² 

[1] Department of Physiology, Semmelweis University of Medicine, 8., P.O. Box 259, H-1444 Budapest, Hungary;Endocrinology and Reproduction Research Branch, National Institute of Child Health and Human Development, National Institute of Health, Bethesda, MD 20892-4510, USA;Division of Cardio-Renal Products, Center for Drug Evaluation and Research, Food and Drug Administration, Rockville, MD 20857, USA

关键词: AT1 angiotensin receptor;    Carboxyl-terminal tail;    Inositol phosphate;    Receptor expression;    Ca2+ mobilizing hormone;   

DOI  :  10.1016/S0014-5793(98)00511-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A series of deletion mutants was created to analyze the function of the membrane-proximal region of the cytoplasmic tail of the rat type 1a (AT_1a) angiotensin receptor. In transiently transfected COS-7 cells, the truncated mutant receptors showed a progressive decrease in surface expression, with no major change in binding affinity for the peptide antagonist, [Sar¹,Ile⁸]angiotensin II. In parallel with the decrease in receptor expression, a progressive decrease in angiotensin II-induced inositol phosphate responses was observed. Alanine substitutions in the region 307–311 identified the highly conserved phenylalanine³⁰⁹ and adjacent lysine residues as significant determinants of AT_1a receptor expression.

【 授权许可】

Unknown