FEBS Letters | |
Requirement of membrane‐proximal amino acids in the carboxyl‐terminal tail for expression of the rat AT1a angiotensin receptor | |
Jagadeesh, Gowraganahalli3  Hunyady, László1  Gáborik, Zsuzsanna1  Mihalik, Balázs1  Catt, Kevin J.2  Jayadev, Suman2  | |
[1] Department of Physiology, Semmelweis University of Medicine, 8., P.O. Box 259, H-1444 Budapest, Hungary;Endocrinology and Reproduction Research Branch, National Institute of Child Health and Human Development, National Institute of Health, Bethesda, MD 20892-4510, USA;Division of Cardio-Renal Products, Center for Drug Evaluation and Research, Food and Drug Administration, Rockville, MD 20857, USA | |
关键词: AT1 angiotensin receptor; Carboxyl-terminal tail; Inositol phosphate; Receptor expression; Ca2+ mobilizing hormone; | |
DOI : 10.1016/S0014-5793(98)00511-0 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
A series of deletion mutants was created to analyze the function of the membrane-proximal region of the cytoplasmic tail of the rat type 1a (AT1a) angiotensin receptor. In transiently transfected COS-7 cells, the truncated mutant receptors showed a progressive decrease in surface expression, with no major change in binding affinity for the peptide antagonist, [Sar1,Ile8]angiotensin II. In parallel with the decrease in receptor expression, a progressive decrease in angiotensin II-induced inositol phosphate responses was observed. Alanine substitutions in the region 307–311 identified the highly conserved phenylalanine309 and adjacent lysine residues as significant determinants of AT1a receptor expression.
【 授权许可】
Unknown
【 预 览 】
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