Protein kinase C rapidly up‐regulates the number of AT1 angiotensin receptors on cultured rat intestinal epithelial (RIE‐1) cells

FEBS Letters

Smith, Roger D.¹ 

[1] Department of Clinical Biochemistry, Addenbrooke's Hospital, Cambridge, CB2 2QQ, UK

关键词: Protein kinase C;    Up-regulation;    AT1 angiotensin receptor;    RIE-1 cell;    Intestinal epithelium;    AII;    angiotensin II;    BM;    binding medium;    DMEM;    Dulbecco's modified Eagle's medium;    EGF;    epidermal growth factor;    HEPES;    N-2-hydroxyethylpiperazine-N′-2-ethane-sulphon ic acid;    IGF-I;    insulin-like growth factor type I;    PIP2;    phosphatidylinositol 4;    5 bis-phosphate;    PKC;    protein kinase C;    TPA;    12-O-tetradecanoylphorbol-13-acetate;   

DOI  :  10.1016/0014-5793(94)00584-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Addition of 12-O-tetradecanoylphorbol-13-acetate to RIE-1 rat intestinal epithelial cells stimulated a rapid (mean 3-fold) increase in the subsequent binding of ¹²⁵ I-labelled angiotensin II which was reversed or prevented when cellular protein kinase C was depleted. The increased binding was due, in part, to an up-regulation in the number of AT₁ angiotensin receptors on RIE-1 cells, without any significant change in their binding affinity. Since this rapid up-regulation was independent of receptor synthesis, it may result from an increased availability (to extracellular ligand) of preformed, but previously ‘cryptic’, AT₁ angiotensin receptors.

【 授权许可】

Unknown