FEBS Letters | |
Protein kinase C rapidly up‐regulates the number of AT1 angiotensin receptors on cultured rat intestinal epithelial (RIE‐1) cells | |
Smith, Roger D.1  | |
[1] Department of Clinical Biochemistry, Addenbrooke's Hospital, Cambridge, CB2 2QQ, UK | |
关键词: Protein kinase C; Up-regulation; AT1 angiotensin receptor; RIE-1 cell; Intestinal epithelium; AII; angiotensin II; BM; binding medium; DMEM; Dulbecco's modified Eagle's medium; EGF; epidermal growth factor; HEPES; N-2-hydroxyethylpiperazine-N′-2-ethane-sulphon ic acid; IGF-I; insulin-like growth factor type I; PIP2; phosphatidylinositol 4; 5 bis-phosphate; PKC; protein kinase C; TPA; 12-O-tetradecanoylphorbol-13-acetate; | |
DOI : 10.1016/0014-5793(94)00584-2 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Addition of 12-O-tetradecanoylphorbol-13-acetate to RIE-1 rat intestinal epithelial cells stimulated a rapid (mean 3-fold) increase in the subsequent binding of 125 I-labelled angiotensin II which was reversed or prevented when cellular protein kinase C was depleted. The increased binding was due, in part, to an up-regulation in the number of AT1 angiotensin receptors on RIE-1 cells, without any significant change in their binding affinity. Since this rapid up-regulation was independent of receptor synthesis, it may result from an increased availability (to extracellular ligand) of preformed, but previously ‘cryptic’, AT1 angiotensin receptors.
【 授权许可】
Unknown
【 预 览 】
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