Enhancement of transcriptional activity of T7 RNA polymerase by guanidine hydrochloride

FEBS Letters

Dasgupta, Dipak¹  Das, Mili¹ 

[1]Biophysics Division, Saha Institute of Nuclear Physics, 37 Belgachia Road, Calcutta 700 037, India

关键词: T7 RNA polymerase;    Guanidine hydrochloride;    Processive transcription;    Abortive transcription;   

DOI  :  10.1016/S0014-5793(98)00458-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

T7 RNA polymerase shows an increase in processive transcription in the presence of low concentrations of guanidine hydrochloride (GdnCl) upto 60 mM, which is not observed when the enzyme is treated with urea. Higher concentrations of the denaturant lead to a progressive loss in the processive transcriptional activity of the enzyme. We have attempted to explain the above phenomenon in terms of the structural change in the enzyme. Fluorescence and CD studies suggest that the tertiary structure of the native enzyme undergoes an alteration upon addition of low concentration of guanidine hydrochloride. This is also indicated from the decreased susceptibility of the enzyme to limited proteolysis by trypsin.

【 授权许可】

Unknown