P1,P4‐diadenosine 5′ tetraphosphate induces nitric oxide release from bovine aortic endothelial cells

FEBS Letters

Christensen, Eric F¹  Hilderman, Richard H¹ 

[1] Departments of Microbiology/Molecular Medicine, Biological Sciences, Vascular Research Laboratory, Greenville Hospital System/Clemson University Biomedical Cooperative, and the South Carolina Experiment Station, Clemson University, Clemson, SC 29634-1903, USA

关键词: Nitric oxide;    Receptor;    Signal transduction;    Bovine aortic endothelial cell;   

DOI  :  10.1016/S0014-5793(98)00454-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Since the infusion of P¹,P⁴-diadenosine 5′ tetraphosphate (Ap4A) into animal models induces vasodilation [1, 2], the present study was performed to determine whether Ap4A induces the release of nitric oxide (NO) from endothelial cells. Ap4A induced NO release was 4.2-fold greater than the amount of NO released under basal condition. Ap4A induced NO release was inhibited by N ^G-nitro-l-arginine (L-NNA) and this inhibition was reversed by L-Arg. In addition, EGTA inhibits Ap4A induced NO release. These data are consistent with Ap4A inducing the release of NO from endothelial cells through the activation of endothelial nitric oxide synthase.

【 授权许可】

Unknown