A cosmid and cDNA fine physical map of a human chromosome 13q14 region frequently lost in B‐cell chronic lymphocytic leukemia and identification of a new putative tumor suppressor gene, Leu5

FEBS Letters

van den Berg, Anke⁵  van Everdink, Wim⁵  Yankovsky, Nick¹  Deaven, Larry⁴  Gizatullin, Rinat⁷  Oscier, David⁶  Kapanadze, Bagrat¹  Syomova, Natalia¹  Ibbotson, Rachel⁶  Sulimova, Galina¹  Lehrach, Hans⁸  Brodyansky, Vadim¹  Poltaraus, Andrey²  Zabarovsky, Eugene R.⁷  Corcoran, Martin⁶  Grander, Dan³  Liu, Yie³  Kazakov, Alexey¹  Kashuba, Vladimir⁷  Zelenin, Alexander²  Rasool, Omid³  Syomov, Alexander¹  Einhorn, Stephan³  Fedorova, Ludmila²  Baranova, Anna¹  Buys, Charles⁵ 

[1] Vavilov Institute of General Genetics, 117809 Moscow, Russia;Engelhardt Institute of Molecular Biology, 117894 Moscow, Russia;Research Laboratory of Radiumhemmet, Department of Oncology/Pathology, Karolinska Hospital and Institute, S-171 76 Stockholm, Sweden;LANL MS M 888, Los Alamos, NM 87545, USA;University of Groningen, 9713 AW Groningen, The Netherlands;Royal Bournemouth Hospital, Bournemouth BH7 7DW, UK;MTC and GCR Karolinska Institute, S-104 01 Stockholm, Sweden;MPI für Molekulare Genetik, Ihnestrasse 73, D-14195 Berlin, Germany

关键词: Tumor suppressor gene;    Location;    B-cell chronic lymphocytic leukemia;    Zinc binding;    Human;   

DOI  :  10.1016/S0014-5793(98)00357-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

B-cell chronic lymphocytic leukemia (B-CLL) is a human hematological neoplastic disease often associated with the loss of a chromosome 13 region between RB1 gene and locus D13S25. A new tumor suppressor gene (TSG) may be located in the region. A cosmid contig has been constructed between the loci D13S1168 (WI9598) and D13S25 (H2-42), which corresponds to the minimal region shared by B-CLL associated deletions. The contig includes more than 200 LANL and ICRF cosmid clones covering 620 kb. Three cDNAs likely corresponding to three different genes have been found in the minimally deleted region, sequenced and mapped against the contigged cosmids. cDNA clone 10k4 as well as a chimeric clone 13g3, codes for a zinc-finger domain of the RING type and shares homology to some known genes involved in tumorigenesis (RET finger protein, BRCA1) and embryogenesis (MID1). We have termed the gene corresponding to 10k4/13g3 clones LEU5. This is the first gene with homology to known TSGs which has been found in the region of B-CLL rearrangements.

【 授权许可】

Unknown