Two distinct states of the thylakoid bf complex

FEBS Letters

Klughammer, Christof¹  Schreiber, Ulrich¹  Heimann, Sebastian¹ 

[1] Julius-von-Sachs-Institut für Biowissenschaften, Lehrstuhl Botanik I, Universität Würzburg, Julius-von-Sachs Platz 2, D-97082 Würzburg, Germany

关键词: Cytochrome b-563 reduction;    Cytochrome bf complex;    Q-cycle;    Photosynthesis;    chl;    chlorophyll;    cyt;    cytochrome;    DAD;    diaminodurene;    LHC;    light harvesting complex;    PQ;    plastoquinone;    QN;    plastoquinone reducing site of the cyt bf complex;    QP;    plastoquinol oxidizing site of the cyt bf complex;   

DOI  :  10.1016/S0014-5793(98)00326-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Under normal physiological conditions the state of the cyt bf complex is characterized by rapid reoxidation kinetics of cyt b-563 following flash-illumination. It is known that these kinetics are dramatically slowed down under oxidizing conditions. Here we show that this slow-down of cyt b-563 oxidation is the consequence of a relatively slow (half-time of several minutes) transformation of the cyt bf complex into a distinctly different state (termed state-s). Reversal to the normal state requires strong reductive treatment or light-induced electron transport. The results are in line with a recent model of functional cyt bf dimers [Cramer et al., Annu. Rev. Plant Physiol. Plant Mol. Biol. 47 (1996), 477–508], if it is assumed that state-s reflects the monomeric state of the bf complex.

【 授权许可】

Unknown