Homology between a human apoptosis specific protein and the product of APG5, a gene involved in autophagy in yeast

FEBS Letters

Brady, Ged²  Brunet, Clare L²  Grand, Roger J.A¹  Hammond, Ester M¹  Johnson, Gerald D³  Milner, Anne E³  Parkhill, Julian¹  Gregory, Christopher D³ 

[1] CRC Institute for Cancer Studies, The University of Birmingham, Edgbaston, Birmingham B15 2TA, UK;School of Biological Sciences, Stopford Building, Manchester University, Oxford Road, Manchester M13 9PT, UK;The Department of Immunology, The Medical School, The University of Birmingham, Edgbaston, Birmingham B15 2TT, UK

关键词: Apoptosis specific protein;    Saccharomyces cerevisiae;    Apoptosis;   

DOI  :  10.1016/S0014-5793(98)00266-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Apoptosis specific proteins (ASP) are expressed in the cytoplasm of cultured mammalian cells of various lineages following induction of apoptosis. The cDNA encoding ASP has been cloned from a human expression library and has significant homology to the Saccharomyces cerevisiae APG5 gene which is essential for yeast autophagy. The ASP gene, known as hAPG5, can be transcribed to give mRNAs of 3.3 kbp, 2.5 kbp and 1.8 kbp which are present at comparable levels in viable and apoptotic cells, demonstrating that protein expression must be regulated at the translational level. These data indicate a possible relationship between apoptosis and autophagy and suggest evolutionary conservation in mammalian apoptosis of a degradative process present in yeast.

【 授权许可】

Unknown