Loss of a consensus heparin binding site by alternative splicing of latent transforming growth factor‐β binding protein‐1

FEBS Letters

Kemp, Paul R¹  Metcalfe, James C¹  Öklü, Rahmi¹  Hesketh, T.Robin¹ 

[1] Section of Cardiovascular Biology, Department of Biochemistry, Cambridge University, Tennis Court Road, Cambridge, CB2 1QW, UK

关键词: Latent transforming growth factor-β binding protein;    Heparin binding;    Cysteine rich repeat;    Alternative splicing;    TGF-β;    transforming growth factor-β;    LTBP;    latent transforming growth factor-β binding protein;    LR-PCR;    long range polymerase chain reaction;    EGF;    epidermal growth factor;    CHO;    chinese hamster ovary;    DEPC;    diethyl pyrocarbonate;    VEGF;    vascular endothelial growth factor;    PDGF;    platelet derived growth factor;   

DOI  :  10.1016/S0014-5793(98)00257-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Latent transforming growth factor-β binding protein-1 (LTBP-1), plays an important role in controlling localisation and activation of transforming growth factor-β (TGF-β). We show that alternative splicing generates a form of mRNA which lacks bases 1277–1435 (termed LTBP-1Δ53). The 53 amino acids encoded by these bases include the eighth cysteine of the first cysteine repeat and a consensus heparin binding sequence. Sequencing of genomic clones showed that alternative splicing resulted from the use of an intra-exonic 3′ splice acceptor site. The loss of the heparin binding site implies that LTBP-1Δ53 will bind to the extracellular matrix less efficiently than LTBP-1.

【 授权许可】

Unknown