Rapid and specific reactive oxygen species generation via NADPH oxidase activation during Fas‐mediated apoptosis

FEBS Letters

Hirabayashi, Yoshio²  Ono, Yasushi¹  Suzuki, Yoshihiro¹ 

[1] Department of Microbiology, Nihon University School of Medicine, Oyaguchikami-machi, Itabashi-ku, Tokyo 173, Japan;Laboratory for Cellular Glycobiology, Frontier Research Program, The Institute of Chemical and Physical Research (RIKEN), Saitama, Japan

关键词: Fas;    Apoptosis;    Reactive oxygen species;    NADPH oxidase;   

DOI  :  10.1016/S0014-5793(98)00228-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

To determine Fas-induced reactive oxygen species (ROS) generation in cells sensitive to Fas-mediated apoptosis, chemiluminescence, a strong indicator of ROS generation, was monitored after stimulation with agonistic anti-Fas. Fas ligation resulted in a rapid and sustained ROS generation. The generation reached a maximum within 5 min and was still observed at least 40 min after the stimulation. No significant ROS generation was observed under conditions where apoptosis was absent. Furthermore, an NADPH oxidase inhibitor, diphenylene iodonium, prevented Fas-mediated ROS generation, suggesting that Fas induces ROS generation via stimulation of the oxidase system.

【 授权许可】

Unknown