Nitric oxide (NO) disrupts specific DNA binding of the transcription factor c‐Myb in vitro

FEBS Letters

Brendeford, Elen M¹  Andersson, Kristin B¹  Gabrielsen, Odd S¹ 

[1] Department of Biochemistry, University of Oslo, P.O. Box 1041 Blindern, N-0316 Oslo 3, Norway

关键词: Myb;    DNA binding;    Avian myeloblastosis virus;    Nitric oxide;    Redox;   

DOI  :  10.1016/S0014-5793(98)00196-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In an attempt to elucidate signal transduction pathways which may modulate DNA binding of the transcription factor c-Myb, we investigated whether c-Myb could be a target for the signaling molecule nitric oxide (NO) in vitro. NO-generating agents severely inhibited specific DNA binding of the c-Myb minimal DNA-binding domain R₂R₃. This inhibition was readily reversible upon treatment with excess DTT. A redox-sensitive cysteine (C130) was required for this NO sensitivity. Moreover, a DNA-binding domain carrying two of the avian myeloblastosis virus (AMV)-specific mutations (L106H, V117D) appeared to be less sensitive to S-nitrosylation than the wild-type c-Myb. This difference in NO sensitivity may influence the regulation of wild type versus AMV v-Myb protein function.

【 授权许可】

Unknown