FEBS Letters | |
Nitric oxide (NO) disrupts specific DNA binding of the transcription factor c‐Myb in vitro | |
Brendeford, Elen M1  Andersson, Kristin B1  Gabrielsen, Odd S1  | |
[1] Department of Biochemistry, University of Oslo, P.O. Box 1041 Blindern, N-0316 Oslo 3, Norway | |
关键词: Myb; DNA binding; Avian myeloblastosis virus; Nitric oxide; Redox; | |
DOI : 10.1016/S0014-5793(98)00196-3 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
In an attempt to elucidate signal transduction pathways which may modulate DNA binding of the transcription factor c-Myb, we investigated whether c-Myb could be a target for the signaling molecule nitric oxide (NO) in vitro. NO-generating agents severely inhibited specific DNA binding of the c-Myb minimal DNA-binding domain R2R3. This inhibition was readily reversible upon treatment with excess DTT. A redox-sensitive cysteine (C130) was required for this NO sensitivity. Moreover, a DNA-binding domain carrying two of the avian myeloblastosis virus (AMV)-specific mutations (L106H, V117D) appeared to be less sensitive to S-nitrosylation than the wild-type c-Myb. This difference in NO sensitivity may influence the regulation of wild type versus AMV v-Myb protein function.
【 授权许可】
Unknown
【 预 览 】
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RO201912020305694ZK.pdf | 199KB | download |