Molecular cloning of a cDNA encoding a pollen extracellular protein as a potential source of a pollen allergen in Brassica rapa

FEBS Letters

Okada, Takashi¹  Toriyama, Kinya¹  Hanaoka, Kazunori¹  Watanabe, Masao¹ 

[1] Laboratory of Plant Breeding, Faculty of Agriculture, Tohoku University, Tsutsumidori-Amamiyamachi 1-1, Aoba-ku, Sendai 981-8555, Japan

关键词: Anther;    Brassica rapa;    cDNA cloning;    Immunoglobulin E;    Lipid transfer protein;    Pollen allergen;   

DOI  :  10.1016/S0014-5793(98)00174-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A polyclonal antiserum was raised against the extracellular pollen proteins of Brassica rapa and used for screening the expression cDNA libraries made from immature anthers. We obtained five groups of cDNA clones, including cDNAs similar to PCP1, thioredoxin, and lipid transfer protein (LTP). Recombinant protein of the cDNA clone showing sequence similarity to LTP was demonstrated to bind IgE of a patient allergic to Brassica pollen. The cDNA clone reported here, therefore, represents a novel pollen allergen of Brassica rapa.

【 授权许可】

Unknown