FEBS Letters | |
cDNA cloning, sequence analysis and allergological characterization of Par j 2.0101, a new major allergen of the Parietaria judaica pollen | |
Colombo, Paolo1  Porcasi, Rossana1  Geraci, Domenico1  Locorotondo, Giovanni1  Duro, Giovanni1  Costa, Maria Assunta1  Cocchiara, Roberta1  Mirisola, Mario G.1  Izzo, Vincenzo1  Di Fiore, Renata1  | |
[1] Istituto di Biologia dello Sviluppo, CNR, Via Archirafi 20, 90123 Palermo, Italy | |
关键词: cDNA cloning; Allergy; Immunoglobulin E; Parietaria judaica; Pj; Parietaria judaica; IgE; immunoglobulin E; RAST; radioallergosorbent test; cDNA; DNA complementary to mRNA; PCR; polymerase chain reaction; | |
DOI : 10.1016/S0014-5793(96)01328-2 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
A clone (P2) coding for an allergen of Parietaria judaica (Pj) pollen has been isolated and sequenced from a cDNA library in lambda ZAP using a pool of 23 sera from Pj-allergic patients. The clone contained an insert of 622 nucleotides with an open reading frame of 133 amino acids (aa) and a putative signal peptide of 31 aa giving a deduced mature processed protein of 102 aa with a molecular mass of 11 344 Da. The expressed recombinant protein, named rPar j 2.0101, was a major allergen since it reacted with IgE of 82% (23/28) of the sera of Pj-allergic subjects analyzed. It was shown to be a new allergen since (i) the amino acid sequence homology with the already reported recombinant allergen Par j 1.0101 was 45% and (ii) there was no cross-inhibition between rPar j 2.0101 and rPar j 1.0101. In addition, rPar j 2.0101 inhibited 35% of the specific IgE for 10–14 kDa native allergens and preincubation of sera from Pj-allergic patients with both rPar j 2.0101 and rPar j 1.0101 fully abolished the IgE recognition of the 10–14 kDa native allergen region, suggesting that these two allergens contributed to the region.
【 授权许可】
Unknown
【 预 览 】
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