cDNA cloning, sequence analysis and allergological characterization of Par j 2.0101, a new major allergen of the Parietaria judaica pollen

FEBS Letters

Colombo, Paolo¹  Porcasi, Rossana¹  Geraci, Domenico¹  Locorotondo, Giovanni¹  Duro, Giovanni¹  Costa, Maria Assunta¹  Cocchiara, Roberta¹  Mirisola, Mario G.¹  Izzo, Vincenzo¹  Di Fiore, Renata¹ 

[1] Istituto di Biologia dello Sviluppo, CNR, Via Archirafi 20, 90123 Palermo, Italy

关键词: cDNA cloning;    Allergy;    Immunoglobulin E;    Parietaria judaica;    Pj;    Parietaria judaica;    IgE;    immunoglobulin E;    RAST;    radioallergosorbent test;    cDNA;    DNA complementary to mRNA;    PCR;    polymerase chain reaction;   

DOI  :  10.1016/S0014-5793(96)01328-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A clone (P2) coding for an allergen of Parietaria judaica (Pj) pollen has been isolated and sequenced from a cDNA library in lambda ZAP using a pool of 23 sera from Pj-allergic patients. The clone contained an insert of 622 nucleotides with an open reading frame of 133 amino acids (aa) and a putative signal peptide of 31 aa giving a deduced mature processed protein of 102 aa with a molecular mass of 11 344 Da. The expressed recombinant protein, named rPar j 2.0101, was a major allergen since it reacted with IgE of 82% (23/28) of the sera of Pj-allergic subjects analyzed. It was shown to be a new allergen since (i) the amino acid sequence homology with the already reported recombinant allergen Par j 1.0101 was 45% and (ii) there was no cross-inhibition between rPar j 2.0101 and rPar j 1.0101. In addition, rPar j 2.0101 inhibited 35% of the specific IgE for 10–14 kDa native allergens and preincubation of sera from Pj-allergic patients with both rPar j 2.0101 and rPar j 1.0101 fully abolished the IgE recognition of the 10–14 kDa native allergen region, suggesting that these two allergens contributed to the region.

【 授权许可】

Unknown