Fuel oxidation in skeletal muscle is increased by nitric oxide/cGMP – evidence for involvement of cGMP‐dependent protein kinase

FEBS Letters

Young, Martin E¹  Leighton, Brendan¹ 

[1] Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK

关键词: Cyclic guanosine-3′;    5′-monophosphate-dependent protein kinase;    Skeletal muscle;    Nitric oxide;    Fuel utilization;    Contraction;    Cyclic guanosine-3′;    5′-monophosphate;    cGMP;    cyclic guanosine-3′;    5′-monophosphate;    EDL;    extensor digitorum longus;    NO;    nitric oxide;    NOS;    nitric oxide synthase;    PKG;    cGMP-dependent protein kinase;    SNP;    sodium nitroprusside;   

DOI  :  10.1016/S0014-5793(98)00143-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The cyclic guanosine-3′,5′-monophosphate (cGMP) analogue, 8-bromo-cGMP (1 mM), increased glucose oxidation in isolated soleus muscle. The nitric oxide (NO) donor, sodium nitroprusside (SNP) (15 mM), increased glucose, pyruvate, palmitate and leucine oxidation. Removal of extracellular Ca²⁺ did not affect SNP-stimulated glucose oxidation (or other glucose utilization parameters), thus eliminating the influx of Ca²⁺ as a mechanism for the increases. The guanylate cyclase inhibitor, LY-83583 (10 μM), inhibited SNP-stimulated palmitate oxidation and activation of cGMP-dependent protein kinase (PKG). Activation of PKG might supersede any inhibitory effects of NO on respiration to stimulate metabolic fuel oxidation in skeletal muscle.

【 授权许可】

Unknown