Molecular cloning and tissue expression of porcine β‐defensin‐1

FEBS Letters

Shi, Jishu¹  Zhang, Guolong²  Wu, Hua²  Ganz, Tomas¹  Blecha, Frank²  Ross, Christopher R² 

[1] Department of Medicine, UCLA School of Medicine, Los Angeles, CA 90095, USA;Department of Anatomy and Physiology, Kansas State University, 1600 Denison Ave., VMS 228, Manhattan, KS 66506, USA

关键词: Antimicrobial peptide;    Defensin;    Pig;   

DOI  :  10.1016/S0014-5793(98)00134-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Beta-defensins constitute an emerging family of cysteine-rich antimicrobial peptides, which are particularly prominent at mucosal epithelial sites in mammals. Here we report the identification of a novel β-defensin from porcine tissues, porcine β-defensin-1 (pBD-1). The cDNA sequence of pBD-1 encoded a 64 amino acid prepro-peptide, which contained the β-defensin consensus sequence of six invariantly spaced cysteine residues. Northern blot analysis showed that pBD-1 was expressed abundantly in tongue epithelia and that the expression was regulated developmentally. Using RT-PCR, pBD-1 mRNA was detected throughout the respiratory and digestive tracts and also in thymus, spleen, lymph node, brain, liver, kidney, urinary bladder, testis, skin, heart, muscle, bone marrow, peripheral blood neutrophils, alveolar macrophages, and umbilical cord. The wide expression of pBD-1 suggests that this endogenous peptide antibiotic may contribute to both mucosal and systemic host defenses in pigs, which may have implications for the use of porcine tissues and organs in xenotransplantation.

【 授权许可】

Unknown