Son1p is a component of the 26S proteasome of the yeast Saccharomyces cerevisiae

FEBS Letters

Yokosawa, H¹  Fujimuro, M¹  Tanaka, K³  Toh-e, A² 

[1] Department of Biochemistry, Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo 060, Japan;Department of Biological Sciences, Graduate School of Science, University of Tokyo, Hongo, Tokyo 113-0033, Japan;The Tokyo Metropolitan Institute of Medical Science, 3-8-12 Honkomagome, Tokyo 113, Japan

关键词: Proteasome;    SON1;    Synthetic-lethal;    Saccharomyces cerevisiae;    DTT;    dithiothreitol;    SDS;    sodium dodecylsulfate;    PAGE;    polyacrylamide gel electrophoresis;    Suc-LLVY-MCA;    succinyl-Leu-Leu-Val-Tyr-4-methylcoumaryl-7-amide;   

DOI  :  10.1016/S0014-5793(98)00084-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A son1 mutant was isolated as a mutant showing synthetic lethality with nin1-1 which is defective in the p31 component of the regulatory subunit of the yeast 26S proteasome. son1Δ showed a synthetic effect with sen3Δ and sun1Δ, both components of the 26S proteasome, and with cdc28-1N. The 26S proteasome was partially purified from the wild type yeast. The FPLC fractions were analyzed by Western blotting using anti-Son1p antibody and antibodies against some authentic subunits of the 26S proteasome, and we found that Son1p co-migrated with components of the 26S proteasome. The 26S proteasome containing fraction was immunoprecipitated with anti-Son1p antibody. The resultant precipitate contained Nin1p, Sun1p, TBP1, and the 20S proteasome. Combining genetic and biochemical results together, we concluded that Son1p is a component of the yeast 26S proteasome.

【 授权许可】

Unknown