Selective induction of CD8+ T cell functions by single substituted analogs of an antigenic peptide: distinct signals for IL‐10 production

FEBS Letters

Kakehi, Masahiro¹  Kohyama, Masako¹  Totsuka, Mamoru¹  Hachimura, Satoshi¹  Kaminogawa, Shuichi¹  Hisatsune, Tatsuhiro¹ 

[1] Department of Applied Biological Chemistry, The University of Tokyo, 1-1-1, Yayoi, Bunkyo-ku, Tokyo 113, Japan

关键词: Altered peptide ligand;    CD8+ T lymphocyte;    Interleukin 10;    Interferon γ;    Cytotoxicity;    APC;    antigen-presenting cell;    Bt2cAMP;    N 6;    O 2-dibutyryl cyclic adenosine 3′;    5′-monophosphate;    IFN-γ;    interferon-γ;    IL;    interleukin;    MHC;    major histocompatibility complex;    TCR;    T cell receptor;   

DOI  :  10.1016/S0014-5793(98)00082-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The CD8⁺ T cell clone 5F1 produces interleukin 10 (IL-10) and interferon γ (IFN-γ) in response to stimulation with a peptide corresponding to region 142–149 of bovine α_s1-casein (p142–149). Ninety analog peptides derived from p142–149 with single amino acid substitutions of putative T cell receptor contact residues were prepared to examine whether production of IL-10 and IFN-γ by 5F1 can be altered by stimulation with these peptides. We found that some peptides triggered only IL-10 production whereas others induced production of IFN-γ alone or both of these cytokines. Peptides inducing IFN-γ production triggered both cytotoxicity and a proliferative response, whereas peptides inducing production of IL-10 but not IFN-γ triggered neither of these responses. Our results clearly demonstrate that the signaling pathway required for IL-10 production in CD8⁺ T cells differs from that required for IFN-γ production. The distinct cellular signals for IL-10 production appear to be independent of those for cytotoxicity and the proliferative response of CD8⁺ T cells.

【 授权许可】

Unknown