The selective activation of the cardiac sarcolemmal sodium‐calcium exchanger by plasmalogenic phosphatidic acid produced by phospholipase D

FEBS Letters

Hale, Calvin C²  Hsu, Fong F¹  Ebeling, Eric G²  Ford, David A³ 

[1] Department of Medicine, Washington University, St. Louis, MO, USA;Department of Veterinary Biomedical Sciences, Dalton Cardiovascular Research Center, University of Missouri-Columbia, Columbia, MO, USA;Department of Biochemistry and Molecular Biology, St. Louis University Health Sciences Center, 1402 S. Grand Boulevard, St. Louis, MO 63104, USA

关键词: Plasmalogen;    Sodium-calcium exchange;    Myocardium;    Phosphatidic acid;    Phospholipase D;    FMOC;    9-fluorenylmethoxy-carbonyl;    GPC;    sn-glycero-3-phosphocholine;    GPE;    sn-glycero-3-phosphoethanolamine;    PIP2;    phosphatidylinositol-4;    5-bisphosphate;    16:0-18:1 plasmenylcholine (PlasCho);    1-O-hexadec-1′-enyl-2-octadec-9′-enoyl-GPC;    16:0-18:1 phosphatidylcholine (PhosCho);    1-hexadecanoyl-2-octadec-9′-enoyl-GPC;    16:0-20:4 plasmenylethanolamine (PlasEtn);    1-O-hexadec-1′-enyl-2-eicosatetra-5′;    8′;    11′;    14-enoyl-GPE;    16:0-20:4 phosphatidylethanolamine (PhosEtn);    1-hexadecanoyl-2-eicosatetra-5′;    8′;    11′;    14-enoyl-GPE;    di-20:0 phosphatidylcholine;    1;    2-dieicosanoyl-GPC;   

DOI  :  10.1016/S0014-5793(98)00017-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Since plasmalogens are the predominant phospholipid of cardiac sarcolemma, the activation of the sodium-calcium exchanger by either plasmenylethanolamine or plasmalogenic phosphatidic acid generated by phospholipase D was explored. Sodium-calcium exchange activity was 7-fold greater in proteoliposomes comprised of plasmenylethanolamine compared to proteoliposomes comprised of only plasmenylcholine. Phospholipase D treatment of proteoliposomes resulted in 1 mol % conversion of plasmenylcholine or phosphatidylcholine to their respective phosphatidic acid molecular species with a concomitant 8-fold or 2-fold activation of sodium-calcium exchange activity, respectfully. Thus, phospholipase D-mediated hydrolysis of plasmalogens to phosphatidic acid may be an important mechanism for the regulation of the sodium-calcium exchanger.

【 授权许可】

Unknown