FEBS Letters | |
Calcium is sufficient but not necessary for activation of sheep platelet cytosolic phospholipase A2 | |
Zupan, Lori A.1  Gross, Richard W.1  Kruszka, Kelly K.1  | |
[1] Molecular and Cellular Cardiovascular Biochemistry Section, Washington University School of Medicine, St. Louis, MO 63110, USA | |
关键词: Phospholipase A2; Calcium; Platelet; Arachidonic acid; Plasmalogen; | |
DOI : 10.1016/0014-5793(91)80753-P | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
In this study we demonstrate that: (1) although the major phospholipase A2 present in sheep platelets is activated by calcium ions, it can effectively catalyze hydrolysis of the sn-2 ester linkage in phospholipids in the absence of calcium: (2) expression of calcium-independent phospholipase A2 activity can be induced by NACl utilizing purified (but not crude) cytosolic enzyme; and (3) calcium-independent phospholipase A2 activity is regulated by a reconstitutable cytosolic protein. Collectively, these results underscore the fundamental catalytic differences between extracellular and intracellular calcium-dependent phospholipases A2 and demonstrate that calcium is sufficient, but not necessary, for the activation of this class of intracellular phospholipases A2
【 授权许可】
Unknown
【 预 览 】
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