Secretion, purification and activity of two recombinant pepper endo‐β‐1,4‐glucanases expressed in the yeast Pichia pastoris

FEBS Letters

Ferrarese, L¹  Gattolin, S¹  Trainotti, L¹  Casadoro, G¹ 

[1] Department of Biology, University of Padua, via G. Colombo 3, I-35121 Padua, Italy

关键词: Endo-β-1;    4-glucanase;    Pichia pastoris;    Heterologous expression;    Capsicum annuum;    EGase;    endo-β-1;    4-glucanase;    BSA;    bovine serum albumin;    AOX1;    alcohol oxidase 1;    SDS-PAGE;    sodium dodecyl sulphate-polyacrylamide gel electrophoresis;    PCR;    polymerase chain reaction;    CMC;    carboxymethylcellulose;    ORF;    open reading frame;   

DOI  :  10.1016/S0014-5793(97)01592-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Two pepper endo-β-1,4-glucanases, involved in fruit softening (cCel1) and leaf and flower abscission (cCel2), have been expressed in Pichia pastoris. Secretion was obtained by using either the mouse α-factor signal (cCel1) or the native signal sequence (cCel2). Times for optimal expression of the two proteins were different and cCel2 appeared very sensitive to proteolytic degradation. A one-step purification protocol yielded cCel2 in a pure form, while an additional chromatography step was necessary to purify cCel1. The two recombinant proteins are highly active and able to degrade carboxymethylcellulose in viscometric assays. Moreover, they have both a molecular mass (54 kDa) and an isoelectric point (7.2 for cCel2 and 8.5 for cCel1) equal to those of the native proteins, thus suggesting that post-translational modifications have properly occurred.

【 授权许可】

Unknown