Expression of the potassium channel KV3.4 in mouse skeletal muscle parallels fiber type maturation and depends on excitation pattern

FEBS Letters

Bartsch, Jörg W¹  Vullhorst, Detlef¹  Jockusch, Harald¹  Klocke, Rainer¹ 

[1] Developmental Biology Unit, W7, University of Bielefeld, D-33501 Bielefeld, Germany

关键词: KV3.4;    Raw3;    Denervation;    Myotonia;    Reverse transcription polymerase chain reaction;   

DOI  :  10.1016/S0014-5793(97)01577-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We report the detailed expression pattern of the voltage-dependent potassium channel KV3.4 (rat homologue, Raw3) in mouse skeletal muscle. Using semi-quantitative RT-PCR, we show that its expression is detectable at embryonic day 17 and rises to adult levels within 2 weeks after birth. Expression is fiber type-dependent, with mRNA levels being 5–6-fold lower in the mixed slow/fast soleus muscle than in the fast tibialis anterior and extensor digitorum longus muscles. Fast muscles from myotonic mice exhibit low KV3.4 mRNA levels similar to those of wild-type soleus. In denervated extensor digitorum longus, KV3.4 expression declines to perinatal levels. We conclude that KV3.4 expression in mouse skeletal muscle is regulated by the pattern of excitation.

【 授权许可】

Unknown