Expression of chloride channel 1 mRNA in cultured myogenic cells: a marker of myotube maturation

FEBS Letters

Bardouille, Claudia¹  Vullhorst, Detlef¹  Jockusch, Harald¹ 

[1] Developmental Biology Unit, W7, University of Bielefeld, D-33501 Bielefeld, Germany

关键词: Skeletal muscle development;    Cell culture;    Chloride channel 1;    Myotonia;    Quantitative RT-PCR;   

DOI  :  10.1016/0014-5793(96)01098-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The chloride channel ClC-1 is required to maintain a normal excitability of mature muscle fibers; its blockade leads to hyperexcitability, the hallmark of the disease myotonia. In mouse and rat myotubes, representing the embryonic stage of muscle, ClC-1 mRNA is not detectable by Northern blotting. From neonatal to adult, ClC-1 expression increases at least fourfold. Using RT-PCR and hybridization on cultured myotubes were found ClC-1 mRNA at a level of 0.4–1.1% of that in mature mouse muscle, and ≤0.01% in myoblasts, at stages when desmin mRNA levels are already high. The level of ClC-1 mRNA is thus a sensitive and specific indicator of the maturation of skeletal muscle cells.

【 授权许可】

Unknown