期刊论文详细信息
FEBS Letters
Expression of chloride channel 1 mRNA in cultured myogenic cells: a marker of myotube maturation
Bardouille, Claudia1  Vullhorst, Detlef1  Jockusch, Harald1 
[1] Developmental Biology Unit, W7, University of Bielefeld, D-33501 Bielefeld, Germany
关键词: Skeletal muscle development;    Cell culture;    Chloride channel 1;    Myotonia;    Quantitative RT-PCR;   
DOI  :  10.1016/0014-5793(96)01098-8
学科分类:生物化学/生物物理
来源: John Wiley & Sons Ltd.
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【 摘 要 】

The chloride channel ClC-1 is required to maintain a normal excitability of mature muscle fibers; its blockade leads to hyperexcitability, the hallmark of the disease myotonia. In mouse and rat myotubes, representing the embryonic stage of muscle, ClC-1 mRNA is not detectable by Northern blotting. From neonatal to adult, ClC-1 expression increases at least fourfold. Using RT-PCR and hybridization on cultured myotubes were found ClC-1 mRNA at a level of 0.4–1.1% of that in mature mouse muscle, and ≤0.01% in myoblasts, at stages when desmin mRNA levels are already high. The level of ClC-1 mRNA is thus a sensitive and specific indicator of the maturation of skeletal muscle cells.

【 授权许可】

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