High concentration of glucose causes impairment of the function of the glutathione redox cycle in human vascular smooth muscle cells

FEBS Letters

Tachi, Yoichi¹  Bannai, Shiro²  Okamura, Naomichi²  Bannai, Chieko¹  Okuda, Yukichi¹  Yamashita, Kamejiro¹ 

[1] Department of Endocrinology and Metabolism, Institute of Clinical Medicine, University of Tsukuba, Tsukuba, Ibaraki 305, Japan;Department of Biochemistry, Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba, Ibaraki 305, Japan

关键词: H2O2;    Glutathione;    Cystine uptake;    High glucose;    Human vascular smooth muscle cell;   

DOI  :  10.1016/S0014-5793(97)01526-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We demonstrated that high glucose reduced H₂O₂ scavenge activity in human vascular smooth muscle cells. In the cells exposed to high glucose, the intracellular glutathione content decreased, although the NADPH content was unchanged. The rate of uptake of cystine, which is a rate-limiting precursor of the glutathione synthesis, decreased in the high glucose group compared with the control group. These decreases were shown to be dependent on glucose concentration. It was suggested that high glucose causes impairment of the function of the glutathione redox cycle in human vascular smooth muscle cells, resulting in reduced H₂O₂ scavenge activity.

【 授权许可】

Unknown