Regulation of Autophagy by High Glucose in Human Retinal Pigment Epithelium

Cellular Physiology and Biochemistry

关键词: High glucose;    Autophagy;    ROS;    ER stress;   

DOI  :  10.1159/000356654

学科分类：分子生物学，细胞生物学和基因

来源: S Karger AG

【 摘 要 】

Background Autophagy is a self-degradative process that is important for balancing sources of energy at critical times in development and in response to nutrient stress. Retinal pigment epithelium (RPE) works as the outer blood retina barrier and is vulnerable to energy stress-induced injury. However, the effect of high glucose treatment on autophagy is still unclear in RPE. Methods Transmission electron microscopy was used to detect the generation of autophagosome. Small interfering RNA (siRNA) and MTT was used to determine the effect of autophagy on cell viability. Western blots and immunohistochemistry were used to detect the expression pattern of autophagic markers, including LC3 and p62. Results High glucose treatment results in a significant increase in the generation of autophagosome and altered expression of LC3 and p62. High glucose-induced autophagy is independent of mTOR signaling, but is mainly regulated via ROS-mediated ER stress signaling. Conclusion In the scenario of high glucose-induced oxidative stress, autophagy may be required for the removal of damaged proteins, and provide a default mechanism to prevent high glucose-induced injury in RPE. © 2014 S. Karger AG, Basel.

【 授权许可】

CC BY-NC-ND